目的:探讨密蒙花总黄酮含药血浆和雄激素受体阻滞剂对泪腺上皮细胞中STAT1的磷酸化蛋白表达的影响。方法:体外分离及培养泪腺上皮细胞。以H2O2诱导大鼠泪腺上皮细胞凋亡,建立雄激素水平下降所致干眼症的细胞凋亡状态。设立空白血浆组、密蒙花总黄酮含药血浆干预组、丙酸睾酮干预组,分别观察各组STAT1的磷酸化蛋白表达情况;并应用雄激素受体阻滞剂氟他胺,考察密蒙花总黄酮的拟雄激素效应。结果:免疫印迹结果表明,含药血浆干预后,密蒙花总黄酮含药血浆干预组中(0.353±0.494)与丙酸睾酮干预组中STAT1的磷酸化蛋白(0.502±0.036)的表达增强,两组间的差异有统计学意义(P<0.01)。各组加入雄激素受体阻滞剂后,各组间(分别是0.268±0.061,0.283±0.106,0.213±0.071)的STAT1的磷酸化蛋白表达间无差异。结论:密蒙花总黄酮含药血浆可促进STAT1的磷酸化表达,并激活STAT1细胞信号传导通路,而产生与丙酸睾酮相同的雄激素效应。

AIM:To explore the effect of Buddleia flavonoids drug-containing plasma and androgen receptor(AR) blocker on the expression of STAT1 phosphoprotein.METHODS:In vitro lacrimal gland epithelial cells were cultivated with H2O2 to establish the dry eye apoptosis state. Blank plasma group,Buddleia officinalis plasma total flavonoids interfere with drug-containing group,and the intervention group of testosterone propionate were set. The expressions of STAT1 phosphoprotein of each group were observed by Western blot and AR blocker flutamide was used to explore the intended androgen effect of Buddleia flavonoids.RESULTS:After the intervention of drug-containing plasma,the expression of STAT1 Phosphoprotein in Buddleja officinalis drug-containing plasma intervention group(0.353±0.494) and testosterone propionate intervention group(0.502±0.036) were enhanced and the differences between the two groups were significant (P<0.01). After using the AR blocker in all groups,the expression of STAT1 phosphoprotein in each group (0.268±0.061,0.283±0.106,0.213±0.071) had no difference.CONCLUSION:Buddleja officinalis drug-containing plasma total flavonoids can promote the expression of STAT1 phosphorylation. 

R285.5

中国国家自然科学基金资助项目(No.30772824)；中国湖南省自然科学基金资助项目(No.07JJ3049)；中国教育部高等学校博士学科点专项基金资助(No.200805410004)；中国湖南省科技厅科研基金资助项目（No.2009FJ3001）；中国2008湖南省研究生创新基金资助项目