AIM:To evaluate the effects of the extract of buddleja officinalis eye drops in basic tears secretory volume,tear film stability,expression of Bax and Bcl-2 in castrated rats with dry eye,and to investigate the therapeutic effects of extract of buddleja officinalis on dry eye caused by gonadal hormones level imbalance. METHODS:Forty-five Wister masculinity rats were divided randomly into 9 groups,including normal group(A1,A2 and A3),model group(B1,B2 and B3),therapy group with buddleja officinalis extract eye drops(C1,C2 and C3). The "1" stood for being fed for 1 mouth,and the "2" stood for being fed for 2 mouths,and the "3" stood for being fed for 3 mouths. The dry eye model was established with orchiectomy on group B,C. Group C were treated with buddleja officinalis extract eye drops. All rats were detected with SⅠt and BUT. Bax,Bcl-2 were checked on immunohistochemistry. RESULTS:The SⅠt value of group C was significantly higher than that of group B(P<0.01) and the BUT value of group C was significantly longer than that of group B(P< 0.01),which indicated the eye drop could significantly keep basic tears secretory volume and tear film stability. The quantity of expression of Bax in acinar epithelial cell and glandular tube cell of group C were significantly higher than that of group B(P<0.01) and the expression of Bcl-2 in acinar cell and glandular tube cell of group C were significantly higher than that of group B too (P<0.01). CONCLUSION:The main components of extract of buddleja officinalis is the flavonoids,which could significantly inhibit happening of dry eye of rat after androgen level lowered and lacrimal gland apoptosis and keep basic tears secretory volume and tear film stability