目的:观察密蒙花提取物滴眼剂对去势所致干眼症雄鼠基础泪液分泌量、泪膜稳定性、泪腺凋亡相关基因蛋白Bax和Bcl-2表达的影响。方法:将45只Wister雄性大鼠随机分为空白组(A1,A2,A3)、模型组(B1,B2,B3)、密蒙花提取物滴眼剂治疗组(C1,C2,C3),共9组,1:饲养1mo,2:饲养2mo,3:饲养3mo,每组5只。B,C组行去势术建立动物模型,C组以密蒙花提取物滴眼剂连续滴眼治疗,对全部实验大鼠行SchirmerⅠ试验、测量泪膜破裂时间,采用免疫组织化学的方法,对大鼠泪腺组织中凋亡相关基因蛋白Bax和Bcl-2进行检测。结果:C组SchirmerⅠ试验测量值明显高于B组(P<0.01),泪膜破裂时间明显长于B组(P<0.01)。泪腺导管及腺泡上皮细胞中Bax阳性表达的细胞数均明显低于B组,而Bcl-2阳性表达的细胞数均明显高于B组(P<0.01)。结论:密蒙花提取物滴眼剂可显著抑制雄激素水平降低后大鼠干眼症的发生,抑制泪腺细胞凋亡,维持泪腺基础分泌量和泪膜的稳定性。

AIM:To evaluate the effects of the extract of buddleja officinalis eye drops in basic tears secretory volume,tear film stability,expression of Bax and Bcl-2 in castrated rats with dry eye,and to investigate the therapeutic effects of extract of buddleja officinalis on dry eye caused by gonadal hormones level imbalance. METHODS:Forty-five Wister masculinity rats were divided randomly into 9 groups,including normal group(A1,A2 and A3),model group(B1,B2 and B3),therapy group with buddleja officinalis extract eye drops(C1,C2 and C3). The "1" stood for being fed for 1 mouth,and the "2" stood for being fed for 2 mouths,and the "3" stood for being fed for 3 mouths. The dry eye model was established with orchiectomy on group B,C. Group C were treated with buddleja officinalis extract eye drops. All rats were detected with SⅠt and BUT. Bax,Bcl-2 were checked on immunohistochemistry. RESULTS:The SⅠt value of group C was significantly higher than that of group B(P<0.01) and the BUT value of group C was significantly longer than that of group B(P< 0.01),which indicated the eye drop could significantly keep basic tears secretory volume and tear film stability. The quantity of expression of Bax in acinar epithelial cell and glandular tube cell of group C were significantly higher than that of group B(P<0.01) and the expression of Bcl-2 in acinar cell and glandular tube cell of group C were significantly higher than that of group B too (P<0.01). CONCLUSION:The main components of extract of buddleja officinalis is the flavonoids,which could significantly inhibit happening of dry eye of rat after androgen level lowered and lacrimal gland apoptosis and keep basic tears secretory volume and tear film stability

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中国国家自然科学基金资助项目(No.30772824);中国教育部高等学校博士学科点专项科研基金资助项目(No.200805410004);中国湖南省自然科学基金资助项目(No.07JJ3049);中国湖南省科技厅科研基金资助项目(No.2009FJ3001);中国2008湖南省研究生创新基金资助项目~~