AIM:To investigate the anti-proliferate effect of amniotic membrane,mitomycin C(MMC) and sodium hyaluronate(Healon) in glaucoma filtering surgery.METHODS:A total of 15 New Zealand white rabbits(30 eyes) underwent trabeculectomy were randomly divided into 3 groups:amniotic membrane group,MMC group and Healon group. All underwent trabeculectomy. After making the scleral flap,the amniotic membrane was placed under the sclera flap to preserve it in the amniotic membrane group. MMC group was placed 0.25g/L MMC 4 minutes under the scleral flap; Healon group was placed sodium hyaluronate under the scleral flap. Postoperative filtering bleb,corneal and the fundus were observed.RESULTS:At the 28th day,tissues around bleb were taken for pathological examination. The majority of the amniotic membrane group and MMC group formed functional filtering bleb,but the amniotic membrane group had no complications and no pathology of connective tissue collagen. CONCLUSION:The anti-proliferative effect of amniotic membrane in glaucoma filtering surgery seems to be better than that of MMC and Healon group,and there is no side effects,so it seems to be a safe and effective anti-proliferation method,and worth promoting.