目的:探讨青光眼滤过术中羊膜、丝裂霉素(mitomycin C,MMC)和透明质酸(Healon)抗增殖作用。方法:健康同种系新西兰大白兔15只30眼随机选5只为1组,共3组:羊膜组、MMC组和Healon组。均行小梁切除术,在作巩膜瓣后,羊膜组在巩膜瓣下放置保存羊膜;MMC组在巩膜瓣下放置0.25g/L的MMC海绵片4min;Healon组在巩膜瓣下放置透明质酸钠。术后观察滤过泡、角膜和眼底情况。结果:术后28d取滤过泡周围组织作病理检查,羊膜组、MMC组多数形成功能性滤过泡,但羊膜组无任何并发症,病理未见结缔组织胶原化。结论:羊膜在青光眼滤过术中抗增殖作用较MMC,Healon效果好,无副作用,是一种安全有效的抗增殖方法,值得推广。

AIM:To investigate the anti-proliferate effect of amniotic membrane,mitomycin C(MMC) and sodium hyaluronate(Healon) in glaucoma filtering surgery.METHODS:A total of 15 New Zealand white rabbits(30 eyes) underwent trabeculectomy were randomly divided into 3 groups:amniotic membrane group,MMC group and Healon group. All underwent trabeculectomy. After making the scleral flap,the amniotic membrane was placed under the sclera flap to preserve it in the amniotic membrane group. MMC group was placed 0.25g/L MMC 4 minutes under the scleral flap; Healon group was placed sodium hyaluronate under the scleral flap. Postoperative filtering bleb,corneal and the fundus were observed.RESULTS:At the 28th day,tissues around bleb were taken for pathological examination. The majority of the amniotic membrane group and MMC group formed functional filtering bleb,but the amniotic membrane group had no complications and no pathology of connective tissue collagen. CONCLUSION:The anti-proliferative effect of amniotic membrane in glaucoma filtering surgery seems to be better than that of MMC and Healon group,and there is no side effects,so it seems to be a safe and effective anti-proliferation method,and worth promoting.

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