目的:观察地塞米松(dexamethasone,Dxm)对兔玻璃体积血后视网膜病理变化的影响。方法:白兔24只,随机分为实验对照组(A),积血组(B)和Dxm干预组(C),每组8只。A组玻璃体内注入生理盐水0.2mL,B组、C组玻璃体内注入自体血0.2mL;注入后1,24,48h,A和B组给予生理盐水3mL/kg,C组给予Dxm3mg/kg。分别于造模后3,7,14,21d随机处死每组中的2兔4眼观察视网膜病理变化。结果:A组所有时间点,B组3,7d,C组3,7,14d视网膜形态正常。B组14d神经节细胞、内丛状层水样变性;21d视网膜内界膜不完整,内、外核层细胞和光感受器内、外节水样变性。C组21d神经节细胞染色变淡,内丛状层、内核层水样变性。结论:玻璃体积血可引起视网膜细胞水样变性,Dxm可缓解玻璃体积血后视网膜水样变性。

AIM:To observe the effect of dexamethasone (Dxm) on pathological changes of rabbit retina after vitreous hemorrhage. METHODS:Twenty-four white rabbits were randomly divided into normal saline control group(A),vitreous hemorrhage group(B) and Dxm treatment group(C),each with 8 rabbits. Group A was intravitreally injected 0.2mL physiological saline. Group B and group C were intravitreally injected 0.2mL autologous blood. Group A and B were given physiological saline 3mL/kg and group C was given Dxm 3mg/kg at 1st,24th and 48th hour after injection. Two rabbits (4 eyes) in each group were randomly killed on the 3rd,7th,14th and 21st day after animal model was established.RESULTS:Group A at all time points,group B on the 3rd,7th day and group C on the 3rd,7th and 14th day were retinal eumorphism. In group B,retinal ganglion cells and inner nuclear layer cell showed hydropic degeneration on 14th day and it was found that not only inner limiting membrane became thick and injury,but also retinal ganglion cells,inner nuclear layer,outer nuclear layer,inner and outer segments of photoceptor showed hydropic degeneration on 21st day. In group C,it was found retinal ganglion cells,inner nuclear layer showed hydropic degeneration on 21st day. CONCLUSION:Vitreous hemorrhage can cause hydropic degeneration of retina. Dxm can relieve the hydropic degeneration of retinal cells following vitreous hemorrhage.

R776.4