目的:探讨中药青光安对兔实验性急性高眼压后视网膜组织结构及细胞凋亡的影响。方法:将49只家兔随机分为7组,每组7只。其中A1和A2为青光安组;B1和B2为石斛夜光丸组;C1和C2为模型组;D组为空白对照组。青光安连续灌胃1wk后用前房内灌注生理盐水法制成急性高眼压模型。再连续灌胃1wk和2wk处死动物,用光镜和透射电镜观察视网膜超微结构的变化,光镜观察视网膜细胞凋亡情况。结果:1wk和2wk视网膜组织结构差别不大。模型组和石斛夜光丸组视网膜各层结构紊乱,细胞数量不同程度减少,其凋亡小体数量明显多于青光安组,A组与B,C组比较,差异有非常显著统计学意义(P<0.01)。模型组和石斛夜光丸组的神经节细胞核膜欠清,大多数线粒体肿胀,有固缩现象,粗面内质网扩张;青光安组上述改变不明显。结论:青光安具有保护视网膜组织结构,减少细胞凋亡的作用。

AIM:To explore the effects of qingguangan on retinal structure and apoptosis in rabbit eyes with acute ocluar hypertension.METHODS:Forty-nine rabbits were divided into 7 groups randomly,7 rabbits in each group. Group A1 and A2 belonged qing-guangan group.Group B1 and B2 belonged to eyesight-improving bolus of noble dendrobium group.C1 and C2 were model group.Group D was blank control group.After administrated qingguangan intragastrically for one week rabbits were filled normal saline into anterior chamber to make acute ocular hypertension model. Then after rabbits were administrated qingguangan intragastrically for one week and two weeks all were killed. Retinal ulstructure changes were observed in light microscope and transmission electron microscope,while apoptosis was observed in light microscope.RESULTS:Retinal structures were not different in one week and two weeks. Layers of the retina model group and eyesight-improving bolus of noble dendrobium group were disorder and cell numbers decreased. The number of apoptotic bodies of two groups were more than those of qingguangan group. Ganglion nuclear membrane of two groups were less clear. Most of mitochondria were swelling and there was condensation phenomenon,rough endoplasmic reticulum dilation.But changes above mentioned in qingguangan group were not obvious.CONCLUSION:Qingguangan has the effects of protecting retinal structure and reducing apoptosis.

中国湖南省教育厅科研基金重点资助项目( No.06A052);中国湖南省卫生厅科研基金资助项目(No.204084)~~