目的:观察正常人和配戴软性角膜接触镜(soft contact lens,SCL)人群结膜上皮细胞表面亚临床炎症因子HLA-DR和细胞内黏性分子1型ICAM-1(CD54)的表达改变。方法:采用结膜印迹细胞学(conjunctivali mpression cytology,CIC)收集结膜上皮细胞,流式细胞学检查方法测定正常人及配戴软性角膜接触镜者HLA-DR和CD54的表达水平,计算阳性细胞率。结果:长期配戴软性角膜接触镜(>5a)组及(<5a)组中ICAM-1和HLA-DR的表达较正常人组相比明显增加(PHLADR1<0.01,PICAM-1,1<0.01;PHLADR2<0.01,PICAM-1,2<0.01),,前二者比较CD54有明显变化(P<0.01)而HLA-DR无明显变化(P>0.05)。结论:长期配戴软性角膜接触镜者结膜亚临床炎症因子ICAM-1和HLA-DR明显增加,眼表的亚临床炎症增加。

AIM:To observe the change of inflammation agent production of the conjunctiva HLA-DR and intercellular adhesion molecule type 1 (ICAM-1) (CD54) in normal subjects and in soft contact lens (SCL) wear subjects. METHODS:Conjunctival impression cytology(CIC) were used to collect conjunctival epithelium,and the expression levels of HLA-DR and CD54 in normal subjects and in SCL wear subjects were determined by flow cytometry. The percentage of positive cells was calculated and compared between each group. RESULTS:A significant increase of inflammation markers (HLA-DR and ICAM-1) was observed in the long-term(>5a) and short-term(<5a) SCL group compared with the control group (PHLADR1<0.01,PICAM-1,1<0.01;PHLADR2<0.01,.PICAM-1,2<0.01). For the two SCL groups,ICAM-1 has significant change(P<0.01),but HLA-DR has no significant change(P>0.05 ).CONCLUSION:This study confirms the difference in expression of inflammation markers (HLA-DR and ICAM-1) in SCL and the healthy adult. It demonstrates a significant increase of subclinical conjunctival inflammation in SCL compared with the normal.

R77

中国四川省教育厅2004年重点基金项目(No.329)~~