AIM:To observe the change of inflammation agent production of the conjunctiva HLA-DR and intercellular adhesion molecule type 1 (ICAM-1) (CD54) in normal subjects and in soft contact lens (SCL) wear subjects. METHODS:Conjunctival impression cytology(CIC) were used to collect conjunctival epithelium,and the expression levels of HLA-DR and CD54 in normal subjects and in SCL wear subjects were determined by flow cytometry. The percentage of positive cells was calculated and compared between each group. RESULTS:A significant increase of inflammation markers (HLA-DR and ICAM-1) was observed in the long-term(>5a) and short-term(<5a) SCL group compared with the control group (PHLADR1<0.01,PICAM-1,1<0.01;PHLADR2<0.01,.PICAM-1,2<0.01). For the two SCL groups,ICAM-1 has significant change(P<0.01),but HLA-DR has no significant change(P>0.05 ).CONCLUSION:This study confirms the difference in expression of inflammation markers (HLA-DR and ICAM-1) in SCL and the healthy adult. It demonstrates a significant increase of subclinical conjunctival inflammation in SCL compared with the normal.