目的:观察密蒙花提取物滴眼剂对去势所致干眼症雄鼠基础泪液分泌量、泪膜稳定性、泪腺中雄激素受体表达的影响,探讨密蒙花提取物滴眼剂抗雄激素水平下降所致干眼症的作用机制。方法:将45只Wister雄性大鼠随机分为空白组(A1、A2、A3)、模型组(B1、B2、B3)、密蒙花提取物滴眼剂治疗组(C1、C2、C3),共9组,1代表饲养1mo,2代表饲养2mo,3代表饲养3mo,每组5只,对B、C组行去势术建立动物模型,对C组以密蒙花提取物滴眼剂连续滴眼治疗1mo,对全部实验大鼠行Schirmer I试验、测量泪膜破裂时间,采用流式细胞仪检测泪腺中雄激素受体的表达。结果:C组Schirmer I试验测量值明显高于B组(P<0.01),泪膜破裂时间明显长于B组(P<0.01),显示密蒙花提取物滴眼剂能够显著维持泪腺基础分泌量和泪膜的稳定性;C组流式细胞仪检测的AR阳性表达率明显高于B组,显示密蒙花提取物滴眼剂中的有效成分可能有拟雄激素的效应。结论:密蒙花提取物滴眼剂中主要成分为黄酮类物质,可显著抑制雄激素水平降低后大鼠干眼症的发生,其作用机制可能与黄酮类物质结构与雄激素类似,可以起到拟雄激素效应,从而维持泪腺基础分泌量和泪膜的稳定性。

AIM:To evaluate the effects of the extract of Buddleja officinalis eye drops in basic tears secretory volume,tear film stability,expression of androgen receptors(AR) in castrated rats with dry eye,and to investigate the therapeutic effects of extract of Buddleja officinalis on dry eye caused by gonadal hormones level imbalance.·METHODS:A total of 45 Wistar masculinity rats were divided at random into 9 groups,including normal group(A1,A2 and A3),model group(B1,B2 and B3),therapy group with extract of Buddleja officinalis eye drops(C1,C2 and C3).The "1" stood for being fed for 1 month,and "2" for 2 months,and "3" for 3 months.The dry eye model was established with orchiectomy on group B,C.Group C was treated with Buddleja officinalis extract eye drops for one month.All rats were checked with Schirmer Ⅰ test (SⅠt) and tear film break-up time (BUT).Expression of AR was analyzed by flow cytometer(FCM).·RESULTS:The SⅠt value of group C was significantly higher than that of group B (P<0.01) and the BUT value of group C was significantly longer than that of group B (P<0.01),which indicated the eye drop could significantly keep basic tears secretory volume and tear film stability.And the expression of AR of group C was much higher than that of group B,which showed that available composition of the eye drops maybe display androgen-like activity.·CONCLUSION:The main components of extract of Buddleja officinalis is the flavonoids which could significantly inhibit happening of dry eye of rat after androgen level lowered.Its mechanism is like androgen’s and it could display androgen-like activity to keep basic tears secretory volume and tear film stability.

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中国国家自然科学基金资助项目（No.30772824）；中国教育部高等学校博士学科点专项科研基金资助项目（No.200805410004）；中国湖南省自然科学基金资助项目（No.07JJ3049）；中国湖南省科技厅科研基金资助项目（No.2009FJ3001）；中国2008湖南省研究生创新基金资助项目\[湘财教字（2008）68号\]