AIM:To observe the effect of endothelin-1(ET-1) on the cytoskeleton protein F-actin of cultured human trabecular meshwork (HTM) cells.·METHODS:Cultured HTM cells were randomly divided into four groups:control group(0mol/L),low-dose ET-1(10-9mol/L) treatment group,middle-dose ET-1(10-8 mol/L) treatment group,and high-dose ET-1(10-7 mol/L) treatment group.After treated with ET-1,the expression of cytoskeleton protein F-actin in trabecular meshwork was analyzed with Western-blot and the distribution of F-actin was detected with FITC-Phalloidin probe.·RESULTS:ET-1 dose-dependently and significantly increased F-actin in trabecular meshwork cells.The F-actin stress fiber and periphery actin fiber highly increased and manifested mild reorganization after treated with ET-1;and there were much more cell-to-cell and cell-to-extracellular matrix attachments formation in ET-1 treated HTM cells than that in the untreated HTM cells.·CONCLUSION:ET-1 promoted the expression of cytoskeleton protein F-actin and induced the trabecular meshwork actin cytoskeleton reorganization.