目的:观察脂质体LipofectamineTM(LF)2000介导Bcl-xL基因在人角膜基质细胞中表达的时间规律。方法:Bcl-xL/LF2000载体复合物转染人角膜基质细胞,转染后1～15d,采用定量RT-PCR及流式细胞计数法检测目的基因Bcl-xL表达阳性细胞率,观察目的基因在角膜基质细胞中表达的时间变化规律。结果:RT-PCR及流式细胞计数法均显示:在转染后1d开始检测到Bcl-xL表达阳性细胞,阳性率在转染后3d最高(48.3%),此后逐渐降低,转染后15d有少量细胞仍表达Bcl-xL基因。结论:LF2000能有效介导外源基因Bcl-xL转染人角膜基质细胞。

AIM:To evaluate Bcl-xL gene expression rule in human keratocyte transferred with LipofectamineTM (LF)2000.·METHODS:The transfection efficiency was examined with RT-PCR,flow cytometry 1 day,2,3,5,10,15 days after transfection.·RESULTS:Bcl-xL expression in keratocyte was observed 1 day after transfection,at the top on 3 days(48.3%),and declined thereafter,while on 15 days there were still a little Bcl-xL positive cells.·CONCLUSION:Liposome can transfer Bcl-xL gene into keratocyte.

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中国湖北省自然科学基金资助项目(No.2008CDB214)~~