AIM:To evaluate the efficacy of topical amphotericin B limposome(AmBL) injection in the management of keratomycosis in a rabbit model. METHODS: Forty New Zealand White rabbits model of aspergillus keratitis were randomized into four groups (groups A, B, C and D), with 10 eyes in each group. Group A were treated with 5g/L AmBL, group B 2.5g/L AmBL, group C were treated with 5g/L amphotericin B eye drop (AmBS), group D were treated with normal saline as the control group. After 3 days, treatments were performed every 15 minutes, totally for 4 times, then it changed to six times per day. Eye examinations were performed after 3, 5, 7, 10, 15 days. Quantitation of fungi in the cornea was achieved by culturing homogenates and counting colony-forming units. RESULTS: Corneal edema, corneal infiltration range, hypopyon, and conjunctival hyperemia revealed that after 7, 10 days treatment, group A, B was significantly different compared to the other groups (P<0.01), but not at day 3,5. Corneal new vessels in groups A, B were significantly different compared to groups C, D after 7 days treatment but not different after 10, 15 days. Quantitative cultures in groups A, B were significantly different compared to groups C, D after 7, 10 days treatment. CONCLUSION: Intracameral AmBL injection can effectively inhibit the progress of keratomycosis. 2.5g/L AmBL and 5g/L AmBL are not significantly different in therapeutic effect.