·AIM:To observe the effect of antisense oligonucleotide(ASODN)hybridized epidermal growth factor receptor(EGFR)on migration of human retinal glial(RG)cells.·METHODS:Human RG cells were cultured in vitro.Modified EGFR ASODN was transfected into RG cells by lipofectin.By using an in vitro wound healing model in which a small area of a confluent monolayer of RPE cells was denuded,migration was measured by the number of cells that had entered the denuded area.·RESULTS:The cell migration was inhibited effectively by ASODN with lipofectin at 63.27%.The missense oligonucleotides showed no such effect(P<0.01).·CONCLUSION:EGFR ASODN can inhibit the migration of RG cells.·