AIM:To assess the accuracy and variability of corneal thickness in myopic eyes obtained by ultrasound pachy-meter,OrbscanⅡ system and Pentacam Scheimpflug imaging system. ·METHODS:First,Pentacam system,OrbscanⅡ system and A-scan ultrasonic pachymeter were used to measure the corneal thickness on 56 myopic cases(56 right eyes)undergoing laser in situ keratomileusis(LASIK);Then,the results were compared. ·RESULTS:The value of corneal thickness measured by Pentacam,OrbscanⅡ and A-scan ultrasonic pachymetry was 547.04±3.65μm,535.41±32.93μm and 539.46±29.15μm respectively; there was no significant difference between the results with one-way ANOVA; these three kinds of instruments of measuring corneal thickness had a good linear correlation; Pentacam system had good repeatability in the measurement of corneal thickness. ·CONCLUSIONS:The Pentacam system offers an accep-table method for evaluating corneal thickness,which is useful in corneal refractive surgery.