AIM:To observe the clinical effects of vitrectomy combined with intravitreal injection of vancomycin for endophthalmitis. ·METHODS:We carried out retrospective analysis of endophthalmitis in 30 cases 30 eyes. We observed the culture results of vitreous bacteria and fungi. All cases were injected vancomycin into vacuum vitreous after vitrectomy.We further observed the situation in the eyes,including the best-corrected visual acuity and complications. ·RESULTS:During the follow-up time,the endophthalmictis in 29 eyes (97%) were controlled without retinal complication. One case of 1 eye (3%) was panophthalmitis and removal of eye ball. Visual acuities of 19 eyes (63%) were improved,of 7 eyes(23%)were same,of 4 eyes(13%) were decreased. ·CONCLUSION:Vitrectomy combined with intravitreal injection of vancomycin is effective in treating endo-phthalmitis.