AIM:To investigate the clinical efficacy of and side effects of intravitreal injection of Avastin for retinal vein occlusion(RVO),diabetic retinopathy(DR),age-related macular degeneration(ARMD),central serous chorioretinopathy (CSC),retinal choroidal neovascularization (CNV). METHODS:Totally 400 cases 520 eyes of fundus disease diagnosed by outpatient including RVO,DR,ARMD, CSC,CNV and other retinal patients underwent intravitreal injection of avastin,and once every month. The visual acuity,fundus fluorescein angiography(FFA), intraocular pressure,optical coherence tomography (OCT) were observed before and after the treatment,and the complications were summarized and analyzed. RESULTS:Following up for 3 months to 1 year,467 eyes (89.8%) had a normal intraocular pressure and improved OCT and FFA indicators,and with visual acuity improved above 2 lines,retinal edema and bleeding absorption, neovascularization regression;36 eyes(6.9%) with stable visual acuity and improved fundus situation,no neovascularization or increased intraocular pressure and other complications;17 eyes(3.3%) with visual acuity decreased,including 13 eyes(2.5%) from 0.2 to 0.1; One eye(0.2%) had endophthalmitis,visual acuity decreased from 0.6 to 0.2;One eye(0.2%) occurred central retinal artery occlusion,visual acuity changed from 0.02 to light perception;One eye(0.2%) had vitreous hemorrhage;One eye(0.2%) had retinal detachment; The visual acuity was improved without one eye associated with complications of central retinal artery occlusion;All 20 eyes(3.8%) had transient elevation of intraocular pressure,while the intraocular pressure reduce to normal by symptomatic treatment;One eye underwent trabeculectomy surgery after intravitreal injection of avastin treatment,with wound healing time from 7 to 10 days and low intraocular pressure. CONCLUSION:Intravitreal injection of avastin for RVO, DR,ARMD,CSC,CNV is an effective,safe method,and can improve visual acuity and reduce macular and retinal edema,vitreous and retinal hemorrhage;and promote the absorption of effective regression of retinal and choroidal neovascularization;and can inhibit the red iris changes and the occurrence of neovascular glaucoma.