·AIM:To investigate the accuracy of intraocular pressure(IOP)by noncontact tonometer(NCT),and the correlation between the actual IOP and the NCT value in New Zealand white rabbit eyes in high IOP state.·METHODS:The actual IOP was regulated by the height of the saline column through a 22G intravenous catheter needle punctured into vitreous cavity in rabbit eyes,under general anesthesia.The actual IOP was measured with liquid pressure transducer through a 24G needle inserted into the anterior chamber.The NCT value were taken by NCT.The height of the saline column was adjusted,the actual IOP readings in the pressure transducer was varied from 15 to 45mmHg in steps of 5mmHg.At each pressure level the IOP was measured simultaneously with NCT.The NCT error(actual IOP minus NCT value)was calculated.Readings of the two techniques were compared with t-test.The relationship between the actual IOP and NCT value was analyzed with Pearson correlation,and liner regression analysis was performed in the two variables.·RESULTS:NCT values were all obviously lower than actual ones,and the mean NCT error was 13.65±2.25mmHg.The readings of two measurements were of statistically significant differences(P<0.01).But there was also a significant correlation between two measu-rements(r=0.985,P<0.01),and there was a regression equation for actual IOP,Y=10.875+1.170X(F=2691.389,P<0.01),each 0.855mmHg increase in actual IOP(Y)caused 1mmHg increase in NCT values(X).·CONCLUSION:NCT value underestimate the actual IOP in rabbit eyes,NCT error increases as actual IOP increases.There is a significant correlation between two measurements.It is also necessary that the regression equation be used to calibrate NCT value to get a more accurate one.Measuring IOP with NCT is feasible in rabbit eyes in high IOP state.·