目的:探讨血管紧张素转换酶抑制剂(angiotensin converting enzyme inhibitors,ACEI)对视网膜新生血管(retinal neov-ascularization,RNV)的抑制作用。方法:采用随机对照实验研究。选取7天龄C57BL/6J新生小鼠36只,随机分为2组:高氧组和ACEI组,每组18只。ACEI组和高氧组小鼠建立氧诱导视网膜病变模型,出氧箱后每日分别ip卡托普利10mg/kg和等量生理盐水,连续5d。采用荧光素血管灌注视网膜铺片观察视网膜血管形态学改变;制作视网膜组织切片并进行HE染色计数突破视网膜内界膜的血管内皮细胞核数;采用Western blot检测视网膜血管紧张素II(angiotensin II,Ang II)和血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)蛋白的表达。结果:荧光素血管灌注视网膜铺片:高氧组可见大量新生血管丛,伴明显荧光渗漏,ACEI组较高氧组视网膜新生血管减少,荧光渗漏减轻。突破视网膜内界膜的血管内皮细胞核数:ACEI组较高氧组减少,差异具有统计学意义(P=0.00)。视网膜AngII蛋白表达水平:ACEI组较高氧组下调,差异具有统计学意义(P=0.00)。视网膜VEGF蛋白表达水平:ACEI组较高氧组下调,差异具有统计学意义(P=0.00)。结论:早期应用ACEI可一定程度抑制RNV形成。

AIM:To investigate the effect of angiotensin converting enzyme inhibitors(ACEI)on suppressing retinal neovascularization(RNV).METHODS:It was a random control experimental study.Thirty-six seven-day-old C57BL/6J mice were divided into two groups randomly including hyperoxia group and ACEI group,eighteen in each group.ACEI group and hyperoxia group were exposed to 75% oxygen to establish a model of oxygen-induced retinopathy,and received daily intraperitoneal injections of captopril 10mg/kg and the equal amount of saline respectively for five days continuously after leaving oxygen box.Fluorescent angiography was used to assess the vascular pattern of retina.The proliferative neovascular response was quantified by counting the nuclei of new vessels extending from the retina into the vitreous in cross-sections.Angiotensin II(Ang II)and vascular endothelial growth factor(VEGF)protein levels in retinas were measured by Western blot.RESULTS:Fluorescent angiography presented increasing neovascular tufts with fluorescein leakage in hyperoxia group.Neovascular tufts and fluorescein leakage were decreased in ACEI group compared to the hyperoxia group.The number of endothelial cells of new vessels extending from retina to vitreous were decreased significantly in ACEI group compared with hyperoxia group(P=0.00).The expression of Ang II protein in retinas were decreased significantly in ACEI group compared with hyperoxia group(P=0.00).The expression of VEGF protein in retinas were decreased significantly in ACEI group compared with hyperoxia group(P=0.00).CONCLUSION:Early treatment with ACEI could inhibit RNV to some degree and protect the retinas.

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中国辽宁省自然科学基金资助项目(No.20052089)；中国辽宁省教育厅科研基金资助项目(No.20060994)