AIM:To discuss the methods of retinopathy of prematurity(ROP) screening training using binocular indirect ophthalmoscope-linked(BIO-linked) image system. METHODS:By the use of BIO-linked image system, 481 students attended national continuing education courses of our hospital . The associated knowledge of ROP and the basic operation of BIO-linked image system were taught and the effects of training were analyzed. RESULTS:After training, 84.8% students could master the characteristics of prematurity normal fundus and classical ROP, differences of various common pathological changes of fundus, the methods of laser photocoagulation in a week. These students also mastered the image principles of BIO and the basic screening steps of ROP. And 90.0% students mastered the use of BIO, proceeded screening and sent the patients to the treatment center in three months. There was statistically significant difference between using BIO-linked image system and using routine method for training. CONCLUSION:BIO-linked image system is directly perceived, easily mastered and feasible, which is worth promoting for ROP screening training.