AIM:To evaluate the clinical curative effect of irrigating the anterior chamber with solution of amphotericin B to treat the fungal keratitis. METHODS:Forty-two patients(42 eyes) were included in this study.The patients were randomly divided into two groups. One was experimental group:the treatment included local and systemic application of fluconazole and combination of irrigating the anterior chamber with solution of amphotericin B.The other was control group:local and systemic fluconazole was used,irrigating the anterior chamber with 9g/L sodium chloride injection. Ulcer healing,hypopyon absorption, vision and other complications were observed. RESULTS:The data of ulcer healing of experimental group and control group were compared, the result was P=0.001. The data of hypopyon absorption of experimental group and control group were compared, the result was P=0.003. They had statistical significance.The data of vision of the patients before and after the treatment were compared, the result was P=0.300, the difference had no statistical significance. CONCLUSION:Irrigating the anterior chamber with solution of amphotericin B to treat the fungal keratitis reduces fungal keratitis ulcer healing and hypopyon absorption time and the complications.