AIM:To establish a method for rapid detection of clinical suspect fungal corneal ulcer by reverse transcription-polymerase chain reaction(RT-PCR).METHODS:A pair of oligonucleotide sequences,which was based on the conserved region of 18srRNA shared by medically important conditioned fungi,was used as the general primers to amplify the DNAs from clinical suspect fungal corneal ulcer in a RT-PCR assay,and the result was compared with culture.RESULTS:A 400bp specific DNA product was successfully amplified.The positive rate of fungi culture among 26 clinical specimens was 61.9%,while that of RT-PCR amplification was 73.8%.In addition,the accuracy of PCR method in this study was 83.8%,the sensitivity was 100.0%,and the specialty was 33.3%.CONCLUSION:PCR with the general primers is suitable for rapid detection of fungal corneal ulcer because of quickness and high positive rate.