目的:探讨聚合酶链反应(RT-PCR)快速检测在真菌性角膜溃疡诊断中的价值。方法:以条件致病性真菌18srRNA基因保守区的一对寡核苷酸序列为通用引物,对临床拟诊为真菌性角膜溃疡的42例标本进行RT-PCR检测,并与培养方法进行对比。结果:在400bp处出现DNA扩增带者为阳性。26份临床标本的真菌培养阳性,阳性率为61.9%,而经RT-PCR扩增阳性率为73.8%,PCR扩增准确性为83.8%,敏感性为100.0%,特异性为33.3%。结论:真菌通用引物进行PCR反应检测真菌性角膜溃疡速度快、阳性率高,有助于真菌性角膜溃疡的快速诊断。

AIM:To establish a method for rapid detection of clinical suspect fungal corneal ulcer by reverse transcription-polymerase chain reaction(RT-PCR).METHODS:A pair of oligonucleotide sequences,which was based on the conserved region of 18srRNA shared by medically important conditioned fungi,was used as the general primers to amplify the DNAs from clinical suspect fungal corneal ulcer in a RT-PCR assay,and the result was compared with culture.RESULTS:A 400bp specific DNA product was successfully amplified.The positive rate of fungi culture among 26 clinical specimens was 61.9%,while that of RT-PCR amplification was 73.8%.In addition,the accuracy of PCR method in this study was 83.8%,the sensitivity was 100.0%,and the specialty was 33.3%.CONCLUSION:PCR with the general primers is suitable for rapid detection of fungal corneal ulcer because of quickness and high positive rate.

R772.21