AIM:To study the essentiality of combining use of computer-assisted imaging system of indirect ophthalmoscope and wide-field digital retinal imaging system and analyze the result of two methods in the retinopathy of premature (ROP) screening.METHODS:Using the computer-assisted imaging system of indirect ophthalmoscope and wide-field digital retinal imaging system,the ophthalmologist examined the premature infants regularly in the ophthalmologic clinic,neonate department and neonate intensive care department from the 1st October 2009 to the 31st October 2010.The examination would be finished till infants’ retinal vascularization completed in nasal and temple retina.The ROP infants’ information would be preserved and their eye disease process would be followed up timely.It would be chosen to be operated on for those patients whose disease process developed to the pre-threshold and threshold and be followed up regularly till their ROP were complete regression and stationary.Finally the data of ROP patients were statistically analysed.RESULTS:From the 1st October 2009 to the 31st October 2010,1431 premature infants who were the objects of ROP screening (gestational age :27-35 weeks;birth weight:0.8-2.5kg)were examined and followed up regularly and found 111 ROP cases(7.76%).53 ROP cases were examined only by RetCamⅡ,and 58 ROP cases who were borderline cases were examined with combining use of computer-assisted imaging system of indirect ophthalmoscope and wide-field digital retinal imaging system.67.57% of patients were found their disease regression after follow-up.19 cases’diseases developed to styleⅠ of the pre-threshold and 15 cases developed to or above the threshold,34 cases were done by laser photocoagulation and 31 cases had good outcomes after operation and 2 ROP 4A stage cases had bad outcome.CONCLUSION:The study was found when the premature infants were shorter in their gestational weeks and the lighter in birth weight,their incidence of ROP were higher and their disease extent were more serious,and they needed ROP screening timely and early intervention treatment.Combining use of computer-assisted imaging system of indirect ophthalmoscope and wide-field digital retinal imaging system is helpful to increase the rate of ROP diagnosis,to preserve the object data and to ROP screening training.