AIM:To discuss the expressions and clinical significance of survivin and cyclinB1 in basal cell carcinoma(BCC).METHODS:Twenty surgical excision and biopsy of eyelid BCC samples were collected from the department of pathology of Wuhan Central Hospital and Zhongnan Hospital of Wuhan University in 2002-2009,and also 5 normal tissues around the cancer were acted as controls.All samples were fixed in 40g/L formaldehyde,with routine HE staining,observed under light microscope,and were finally diagnosed by pathological examination.Immunohistochemistry method and image analysis technique were used to detect the expression of survivin and cyclinB1 in the eyelid BCC and cancer adjacent tissues,and the expression of survivin and cyclinB1 in cytoplasm and karyon of each group were quantitatively analyzed by HPIAS-2000 High Definition Colour Pathology Image and Character Report Management System.RESULTS:Survivin and cyclinB1 were highly expressed in eyelid BCC tissues,while lowly expressed in cancer adjacent tissues.The average optical density and positive area rate of survivin and cyclinB1 between eyelid BCC and cancer adjacent tissues showed significant difference by one-way ANOVA.CONCLUSION:The abnormal expression of survivin and cyclinB1 in eyelid BCC might play an important role during the genesis and development of eyelid BCC.