AIM:To investigate the cognitive attitudes of evidence-based medicine(EBM) in medical graduates(major in Eye & ENT and optometry) and to learn barriers to applying EBM in clinical practice in medical graduates.METHODS:Totally 273 medical graduates selecting the EBM courses were surveyed by questionnaires.Statistical analysis was conducted on the items of purpose,interest,knowledge obtaining and application practice of EBM courses.RESULTS:From Grade 2005 to Grade 2007,more and more medical graduates thought that EBM could guide research and improve clinical level.More and more students were willing to continue to participate in EBM programs or courses and focus on EBM information.More and more medical graduates acquired the knowledge of EBM through classroom and seminar.The differrence was statistically significant(P<0.05).The barriers to using EBM in clinical practice included the lack of awareness and effective cases.CONCLUSION:EBM education in our university has begun to popularize in the ophthalmic teaching and been firmly established,but there are still many obstacles.We should strengthen the training of EBM and transform it to the application phase.