AIM:To evaluate the timing of operation and safety of probing of lacrimal passage in neonatal dacryocystitis.METHODS:A retrospective case series study.137 out-patients(165 eyes) with neonatal dacryocystitis were selected from January 2009 to June 2010 in our hospital,and treatment conditions were analyzed.RESULTS:In this study,of the 137 cases of 165 eyes,64 eyes were in children with age≤3 months,in which 16 eyes cured with pressure probing,48 eyes cured with probing one time ;68 eyes were in children with age 3-6 months,in which 4 eyes cured with pressure washing,62 eyes cured with once probing,1 eye cured with twice probing,1 case(1 eye) was not followed up;24 eyes was in children with age of 6-12 months,in which 12 eyes cured with once probing,8 eyes cured with twice probing,2 eyes cured with 3 times probing,2 cases(2 eyes) were not followed up;9 eyes was in children with age of 12-18 months,in which 6 eyes cured with once probing,3 eyes cured with twice probing.CONCLUSION:In neonatal dacryocystitis patients with normal physical development,aged above 2 months,probing of lacrimal passage should be performed as soon as possible which is also safe and feasible.