AIM:To observe the application of compound anisodine injection,hormone impact and supplemental energy comprehensive treatment of adolescents with acute optic neuritis. METHODS:Sixty-five cases (82 eyes) adolescent patients with acute optic neuritis were randomly divided into 2 groups:a treatment group of 33 cases (41 eyes) and a control group of 32 cases (41 eyes). At the same time, as the treatment group and control group were treated with the same systemic medication, the affected eyes in the treatment group were injected with compound anisodine around superficial temporal artery, 1 time a day, 2mL each time, In 14, 30, 60 days,the changes in course time, visual acuity, visual evoked potentials and fundus fluorescein angiography were observed before and after treatment in 2 groups. RESULTS: In 14, 30, 60 days, the efficacy observed in treatment group was higher than that in the control group,the difference was statistically significant (P<0.05). CONCLUSION:Combination with compound anisodine injection beside the superficial temporal artery for adolescents with acute optic neuritis can shorten the disease course, reduce the amount of hormone, and increase the patient’s visual acuity more than traditional hormone impact and supplemental energy comprehensive treatment.