目的：探讨100%浓度自体血清滴眼治疗眼表疾病的疗效。 方法：回顾性分析因各种原因引起的持续角膜上皮缺损或溃疡35例35眼，经常规的药物治疗或联合羊膜移植或板层角膜移植治疗后效果不佳，采用100%浓度自体血清治疗2～6wk，观察其症状及角膜上皮修复情况。 结果：患者35例35眼中，治愈率34.3%，好转率40.0%，无效率25.7%。在病毒性角膜炎和神经营养性角膜炎中治愈率和好转率均为47.6%，95.2%的患者角膜上皮和溃疡在滴用100%自体血清1mo修复。未发生感染及不良反应。 结论：100%浓度自体血清滴眼治疗眼表疾病是安全有效的，可明显促进角膜上皮的修复，对于病毒性角膜炎和神经营养性角膜炎引起的持续角膜上皮缺损或溃疡疗效更佳。

AIM: To evaluate the effectiveness of treating ocular surface disorders using 100% autologous serum eye drops. METHODS: Totally 35 cases (35 eyes) who had persistent corneal epithelial defects or ulcer that were nonresponsive to conventional medical treatment or amniotic membrane transplantation or lamellar keratoplasty were reviewed.These patients were treated with 100% autologous serum eye drops for 2-6 weeks. Its symptoms and corneal epithelial repair were observed. RESULTS:In 35 eyes ,12 eyes (34.3%) healed within 2 weeks,14 eyes (40.0%) healed within 4weeks.But the corneal epithelium of 9 eyes (25.7%)did not recover within 4 weeks. In herpetic keratitis and neurotrophic keratopathy the cure rate and improvement rate were 47.6% respectively, 95.2% healed within 4 weeks by using 100% autologous serum eye drops.No side effects and secondary infections were detected. CONCLUSION: The use of 100% autologous serum eye drops appears to be an effective and safe medical therapy in the treatment of persistent corneal epithelial defects or ulcer especially caused by the herpetic keratitis and neurotrophic keratopathy.