目的：探讨低浓度间断吸氧对糖尿病大鼠血液流变学和视网膜渗漏的影响。方法：健康清洁级雄性SD大鼠随机分为3组：对照组、糖尿病组、糖尿病吸氧组。糖尿病组和糖尿病吸氧组大鼠腹腔内注射链脲佐菌素（STZ）。对照组注射柠檬酸盐缓冲液。糖尿病吸氧组大鼠每天吸入浓度为300mL/L氧气2h,2L/min。造模后2mo采用血液流变仪测定大鼠血液流变学参数,采用Evans蓝作为示踪剂定量检测视网膜中Evans的含量,分析血-视网膜屏障的破坏程度。结果：糖尿病组的全血高切黏度、全血中切黏度、全血低切黏度、高切相对指数、低切相对指数、高切还原黏度、低切还原黏度、红细胞压积、红细胞聚集指数、血浆黏度、卡松黏度和血沉方程K值均较对照组和糖尿病吸氧组高,差异具有统计学意义（均P<0.05）；糖尿病吸氧组的血浆黏度和卡松黏度与对照相近,差异无统计学意义（均P>005）；糖尿病吸氧组的全血高切黏度、全血中切黏度、全血低切黏度、高切相对指数、低切相对指数、高切还原黏度、低切还原黏度、红细胞压积、红细胞聚焦指数、红细胞刚性指数均较对照组和糖尿病组降低,差异具有统计学意义（均P<0.05）。糖尿病组视网膜Evans渗漏量较对照组和糖尿病吸氧组高,糖尿病吸氧组视网膜Evans渗漏量较对照组高,但较糖尿病组低,差异具有统计学意义（均P<0.05）。结论：低浓度间断吸氧可降低糖尿病大鼠血液黏度,增加视网膜血供和氧供,减少视网膜渗漏。

AIM: To investigate the effect of low-concentration intermittent oxygen inhalation on diabetic rats' hemorheology and retinal leakage. METHODS: Healthy and clean male SD rats were randomly divided into three groups: control group, diabetes group, diabetes treated with oxygen inhalation group. The rats in the diabetesgroup and the diabetes treated with oxygen inhalation group were intraperitoneally injected streptozotocin (STZ). The rats in the control group were intraperitoneally injected citrate buffer. The rats in the diabetes treated with oxygen inhalation group inhaled 300mL/L oxygen for 2 hours daily. Two months after modeling, the rats' hemorheological parameters were measured with blood rheometer. The rats' retinal Evans leakage was quantitatively measured and the integrity of the blood ocular barrier was analyzed using Evans blue as a tracer.RESULTS: The high shear viscosity of whole blood, middle shear viscosity of whole blood, low-shear viscosity of whole blood, relative index of high shear, relative index of low shear, high-shear reduced viscosity, low shear reduced viscosity, haematocrit, erythrocyte aggregation index, plasma viscosity, Casson viscosity and K value of erythrocyte sedimentation rate equation of diabetes group were higher than those of control group and diabetes treated with oxygen inhalation group. The differences among three groups were statistically significant (all P<0.05). The plasma viscosity and the Casson viscosity of diabetes treated with oxygen inhalation group were similar to those of control group. The differences were not statistically significant (both P>0.05). The high shear viscosity of whole blood, middle shear viscosity of whole blood, low-shear viscosity of whole blood, relative index of high shear, relative index of low shear, high-shear reduced viscosity, low shear reduced viscosity, haematocrit, erythrocyte aggregation index and erythrocyte rigidity index of diabetes treated with oxygen inhalation group were lower than those of control group and diabetes group. The differences among three groups were statistically significant (all P<0.05).The retinal Evans leakage of diabetes group was higher than that of control group and diabetes treated with oxygen inhalation group. The retinal Evans leakage of diabetes treated with oxygen inhalation group was higher than that of control group, while was lower than that of diabetes group. The differences among three groups were statistically significant (P<0.05).CONCLUSION: Low-concentration intermittent oxygen inhalation can reduce diabetic rats' blood viscosity, increase diabeticrats' retinal blood supply and oxygen supply, and reduce diabetic rats' retinal leakage.

福建省教育厅科技资助项目(No. JB09120);福建省科技平台建设资助项目(No. 2010Y2003)