AIM:To study the application of amniotic membrane transplantation combined with adjustable sutures in trabeculectomy. METHODS:In our hospital from January 2009 to June 2010, 50 cases(54 eyes) with acute angle-closure glaucoma after trabeculectomy were included. After conventional trabeculectomy, a 6mm×7mm amniotic membrane was placed under scleral flap on the scleral bed, scleral flap corners were fixed 2 needles. One or two sides of the scleral flap was fixed with 1or 2 adjustable sutures, knotting in the transparent cornea. BSS formed the anterior chamber. The follow-up was 1.5 years. RESULTS:The postoperative intraocular pressure was well controlled, with a success rate of 89%, conditional successful rate of 7%, failed 4%. CONCLUSION:Amniotic membrane transplantation combined with adjustable suture is a safe, effective method in treatment of acute angle-closure glaucoma.