补肾活血中药对大鼠慢性高眼压模型初级视皮质尼氏小体损害的影响
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中国四川省教育厅2010年重点项目(No.10EA09)


Influence of Bushenhuoxue on primary visual cortex's Nissl bodies damage in rat model of chronic elevated intraocular pressure
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Key Projects of Sichuan Provincial Education Office, China(No.10ZA096)

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    摘要:

    目的:观察补肾活血中药对大鼠慢性高眼压(elevated intraocular pressure,EIOP)模型初级视皮质尼氏小体损害的干预作用,并对其作用机理进行初步探讨。

    方法:采用烙闭上巩膜静脉法,烙闭大鼠3支上巩膜静脉,制作大鼠慢性EIOP模型,随机分为3组:空白组,模型组,给药组。连续灌胃8wk,并于8wk末处死大鼠,观察其对EIOP大鼠眼压(intraocular pressure,IOP),初级视皮质(primary visual cortex,PVC)尼氏小体含量、神经元细胞超微结构影响。

    结果: 本实验采用的烙闭上巩膜静脉的造模方法使大鼠眼压明显升高(P<0.01),与造模前比较差异有显著统计学意义(P<0.01); PVC病理切片半定量分析表明:模型组尼氏小体总面积34941±8482.1·S/μm2、平均光密度152.8±27.97、积分光密度11993±3084.8,与空白组(总面积55742±6348.1·S/μm2、平均光密度304.04±100.1、 积分光密度18219±3548.9)比较均有显著统计学意义(均为P<0.05),模型组Nissl小体总面积、平均光密度、积分光密度与给药组(总面积46406±5989.3·S/μm2,平均光密度251.05±77.41,积分光密度16899±4040.6)比较,差异均有统计学意义(均P<0.05)。

    结论:补肾活血中药通过增强尼氏小体的表达、改善神经元细胞超微结构而促进EIOP大鼠初级视皮质损伤的修复。

    Abstract:

    AIM: To observe the effect of Traditional Chinese Medicine(TCM)of Bushenhuoxue on primary visual cortex(PVC)'s Nissl bodies damage in rat model of chronic elevated intraocular pressure(EIOP), and explore the mechanism of it initially.

    METHODS: The rat model of chronic EIOP was established by unilaterally cauterizing 3 episcleral vessels, then 30 rats were divided into 3 groups randomly: control group, model group, and treatment group. After given drugs or normal saline for 8 weeks, the rats were put to death. The effect of intraocular pressure(IOP), content of Nissl bodies and ultrastructure of neuron cell in the PVC was observed.

    RESULTS: Unilaterally cauterizing episcleral vessels increased IOP of the rat modle obviously, there was significant difference compared with preoperative(P<0.01). Semi-quantitative pathological analysis showed that Nissl body of total area in the model group was(34941±8482.1)S/μm2,mean optical density was(152.8±27.97), integrated optical density was(11993±3084.8), compared with the control group, total area was(55742±6348.1)S/μm2,mean optical density was(304.04±100.1),integrated optical density was(18219±3548.9), there were statistically differences(all P<0.05). There were statistically difference in Nissl body of total area, mean optical density and integrated optical density between model group and treatment group, total area was(46406±5989.3)S/μm2,mean optical density was(251.05±77.41),integrated optical density was(16899±4040.6, all P<0.05).

    CONCLUSION: TCM of Bushenhuoxue can repair PVC's damage in the rat model of chronic EIOP by enhancing expression of Nissl body, improving ultrastructure of neuron cell.

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李翔,郭红建,文晓霞,等.补肾活血中药对大鼠慢性高眼压模型初级视皮质尼氏小体损害的影响.国际眼科杂志, 2012,12(12):2256-2260.

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  • 收稿日期:2012-08-20
  • 最后修改日期:2012-11-05
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