AIM: To evaluate therapeutic effect of the early stage of multiple fresh amniotic membrane transplantation in the treatment of severe ocular surface alkali burn.

METHODS: We study the therapeutic effect of 25 cases(25 eyes)who transplanted with twice or more than twice amniotic membrane in early stage of treatment severe ocular surface alkali burn in our hospital from January 2007 to December 2011 by retrospective analysis. After operation, we observed ocular surface stability, corneal epithelial healing, corneal angiogenesis, and visal activity to further investigate therapeutic effect of the early multiple fresh anmiotic membrane transplantation in the treatment of severe ocular surface alkali burn.

RESULTS: There were 20 eyes amniotic membrane dissolved between 6-14 days after second anmiotic membrane transplant, and the amniotic membrane detached after sutures out in 20 cases(25 eyes). Inflammation of ocular surface was mainly under control, fluorescein sodium described that corneal epithelial was integrated and corneal limbal ischemia was improved basically in these 20 eyes. 4 eyes corneal epithelium still defect more than 2 mm diameter or more, cornea limbal ischemia more than 1/4 quadrant after two amniotic membranes, then did the third transplantation, the eye conserved intact, ocular surface keep stable ultimately. One patient leaved hospital for some reason after twice transplantation induced to follow up failure.

CONCLUSION: Obviously,multiple fresh amniotic membrane transplantation for treatment of severe ocular surface burn in the early stage is useful to preserve ocular surface stable effectively.