方法：回顾分析2009-06/2012-10临床收集的28例28眼板栗刺所致角膜异物患者资料，所有患者均伴有外伤眼角膜局部浸润、水肿，角膜表面浅层溃疡形成。板栗刺异物均深达角膜基质层，但未穿透入前房。对所有患者均行急诊手术，剔除角膜异物，对其中14例患者同时行角膜局部清创联合多层羊膜移植术，作为治疗组； 其他14例患者拒绝行羊膜移植，仅剔除角膜异物，作为对照组。随访至术后3mo，裂隙灯观察角膜上皮愈合时间，并观察术后视力提高程度及并发症的发生率。

结果：治疗组患者角膜上皮平均愈合时间明显较对照组缩短(t=13.6，P<0.05)，手术前后治疗组患者视力提高率明显高于对照组，并发症的发生率明显下降。

结论：对于中深层板栗刺角膜异物合并角膜溃疡的患者，急诊手术剔除角膜异物同时行羊膜移植手术可以促进角膜修复，并预防伤后并发症的发生。

METHODS: Retrospective analysis of 28 cases(28 eyes)with infectious keratitis due to chestnut thorn from June 2009 to October 2012. All patients had the clinical manifestations such as local infiltration, edema and ulcer formation. Chestnut thorn located deeply into corneal stroma, but did not penetrate into the anterior chamber. All patients underwent emergency surgery to remove chestnut thorn, of which 14 patients underwent corneal debridement joint multilayer amniotic membrane transplantation as the treatment group, and the other 14 patients refused amniotic membrane transplantation and had the chestnut thorn removed only as the control group. The corneal epithelial healing time, the degree of improvement of visual acuity and the incidence of complications were compared between the two groups after 3 months.

RESULTS: The corneal epithelial average healing time of the treatment group was significantly shortened compared with the control group(t=13.6, P<0.05), the visual acuity of the treatment group was improved significantly higher than that in the control group, and the incidence of complications of the treatment group was significantly decreased compared with the control group.

CONCLUSION: For the patients with corneal ulcer due to deep chestnut thorn, emergency surgery of corneal debridement joint multilayer amniotic membrane transplantation can promote the repair of the cornea and prevent the occurrence of complications after injury.