Abstract:AIM: To explore the roles of neuronal axon-guidance molecules Slit3 and Robo4 receptor in corneal neovascularization(CNV)by study their expression in neovascularized cornea of rats.
METHODS: CNV models were established by implantation pellets containing basic fibroblast growth factor(bFGF)into corneal stroma. CNV models were measured by biomicroscopy photography. Immunohistochemical staining and imaging analysis system were used to detect the expression of Slit3 and Robo4 in the models after 1, 4, 7, 10 and 14d.
RESULTS: The area of CNV and the expression of Slit3, Robo4 were increased in CNV models compared to that in normal cornea and reached highest level on 7d. And the expression level of Slit3 and Robo4 were significantly correlated with the size of CNV on every time point except 1d(r=0.84-0.91, all P<0.05).
CONCLUSION: The expression of Slit3 and Robo4 may be related to the CNV development. They are potential therapeutical target for CNV.
