AIM: To investigate the changes of electroretinogram in mice with the growth and development of retina.

METHODS: The ERG of 50 mice(50 eyes)of KUNMING at the ages of postnatal 14d(P14), P21, P28, P35 and P56 were measured respectively. The implicit times and amplitudes of b wave of Rod-ERG, a and b waves of Max-ERG, a and b waves of Cone-ERG and O1 and O2 waves of OPs at different ages, as well as amplitude of Flick-ERG, were compared.

RESULTS: The Max-ERG a-waves(the 95%CI were 15.00～18.60, 12.00～15.00, 13.20～14.40, 13.20～15.00, 13.20～15.00, respectively), OPs O1(the 95%CI were 15.00～19.80, 13.80～18.00, 13.20～14.40, 13.80～15.60, 13.80～15.60, respectively)waves shared the implicit times at the different stages, and the Flick-ERG(the 95%CI were 0.97～3.28, 0.85～2.32, 0.91～3.49, 0.94～2.68, 0.98～3.69μV, respectively)shared the amplitudes also. There was no significant difference among the weeks(P>0.05). The implicit times of the Cone-ERG a-waves(the 95%CI were 25.20～55.20, 27.00～40.20, 27.00～38.40, 25.20～43.80, 23.40～37.80, respectively)between P14 and P28 were distinct with statistical difference(P<0.05). The implicit times of Cone-ERG b-waves(the 95%CI were 70.80～88.20, 56.40～78.60, 60.00～75.60, 60.60～87.00, 62.40～81.60ms, respectively)at P14 were statistically different from those at P21 and at P28. The implicit times and amplitudes of Rod-ERG b-waves(the 95%CI were 87.00～114.00, 53.40～73.80, 52.2～63.6, 55.20～71.40, 57.60～67.80ms, and 64.21～195.07, 133.79～355.71, 130.62～355.96, 190.92～448.97, 239.26～462.40μV, respectively), Max-ERG b-waves(the 95%CI were 67.20～107.40, 32.40～54.60, 31.20～36.60, 31.80～42.00, 34.20～41.40ms, and 160.64～344.48, 281.74～590.09, 284.91～716.80, 358.64～737.55, 406.98～810.55μV, respectively), and OPs O2 waves(the 95%CI were 49.8～69.6, 29.40～42.60, 28.80～33.60, 28.80～37.80, 31.20～37.20ms, and 5.43～24.84, 54.38～147.52, 65.55～201.60, 46.33～164.79, 49.07～148.32μV, respectively)at P14 were different from those at other stages, and the amplitudes of OPs O1(the 95%CI were 11.60～21.36, 6.77～53.71, 32.96～76.42, 34.06～70.37, 35.58～63.35μV, respectively)and Cone-ERG b-waves(the 95%CI were 5.10～15.85, 9.61～24.88, 14.96～40.73, 14.87～28.54, 13.83～51.98μV, respectively)were from those at other stages also, and there were significant differences. The O1 wave of OPs had been present at P14, but the second cluster of OPs of one mouse(1/10)had not been obvious at the same time.

CONCLUSION: The experiment confirms that the different waves come from different cells in retina in mice at certain degree. Due to the change of the ERG in the development of mice, so it should be considered that the different development stages will affect the results when measuring the ERG of mice.