方法：选取50只兔，随机分为两组，每组25只，即实验组和对照组，并建立兔角膜碱烧伤模型。实验组在角膜碱烧伤后当天行结膜瓣遮盖术。裂隙灯下观察两组角膜病损恢复情况并照相。采用苏木素-伊红(HE)染色切片后测定两组角膜碱烧伤后不同时间点PMNs的浸润量值。

结果：PMNs在角膜碱烧伤后的3d开始升高，大量浸润角膜，7d略有下降，14d上升达到最大峰值，之后逐渐降低，早期分布在烧伤部位角膜基质内，后期则与溃疡浸润面积相一致。实验组PMNs的计数均明显低于对照组，且角膜碱烧伤后的3、14和21d的差异有统计学意义(P<0.05)。

结论：结膜瓣遮盖治疗重度角膜碱烧伤的疗效确切，角膜的病理损伤和修复与PMNs的浸润密切相关。

METHODS: Rabbit cornea alkali-burned model was made, then 50 rabbits were randomly divided into the experimental group(n=25)and the control group(n=25). At the same time the surgery of conjunctival flap covering was given to rabbits of the experimental group. The condition developing of alkali-burned cornea was observed by slit lamp biomicroscopy, and took photos in two groups. The infiltration of PMNs was identified by hematoxylin eosin(HE)staining in different periods.

RESULTS:The quantity of PMNs increased on the 3d, reached the lower level on 7d, shown a peak on the 14d, then decreased gradually. PMNs level of the experimental group was significantly lower than that in the control group, and the difference of 3, 14 and 21d was significant(P<0.05).

CONCLUSION: During the wound healing process, alkali-burned cornea has close relation with the infiltration of PMNs. The treatment of conjunctival flap covering for the severe alkali-burned cornea was found to have good effect.