目的：探讨不同浓度红景天苷对H₂O₂诱导的人晶状体上皮细胞(human lens epithelium cells，HLEC)氧化损伤的影响。

方法：HLEC传代培养，分为4组：对照组：以正常培养液培养； 氧化损伤组：100μmol/L的H₂O₂作用12h； 红景天苷低浓度组：10μmol/L红景天苷预处理24h后，加入H₂O₂作用12h； 红景天苷高浓度组：100μmol/L红景天苷预处理24h后，加入H₂O₂作用12h。应用MTT法观察红景天苷对HLEC细胞活力的影响； 倒置显微镜下观察并记录各组细胞形态变化； DCFH-DA荧光探针检测胞内ROS含量的变化； 分光光度计检测细胞内超氧化物歧化酶(superoxide dismutase，SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase，GSH-Px)及丙二醛(malondialdehyde，MDA)的含量。

结果：红景天苷能明显抑制H₂O₂诱导的HLEC细胞活力下降，抑制细胞内ROS生成，引起HLEC内SOD、GSH-Px水平的升高及MDA水平的下降。

结论：红景天苷通过降低细胞内MDA产生，提高细胞内SOD、GSH-Px含量抑制H₂O₂诱导的HLEC损伤，提示红景天苷可能在HLEC损伤的治疗中具有保护作用。

METHODS: HLEC were cultured and divided into negative control group: cultured in normal cultivation; oxidative damage group: treated with 100μmol/L H₂O₂ for 12h; Salidroside low concentration group: 10μmol/L salidroside treated for 24h and H₂O₂ treated for 12h; Salidroside high concentration group: 100μmol/L salidroside treated for 24h and H₂O₂ treated for 12h. MTT method was applied to observe the effect of salidroside on HLEC survival rate. Morphological change of each group were observed and recorded under inverted microscope. DCFH-DA fluorescent probe was applied to detect intracellular ROS changes; content of malondialdehyde(MDA), superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in supernatants were detected by pectrophotometer.

RESULTS: Salidroside obviously inhibited H₂O₂-induced HLEC vitality decline, inhibited ROS generation in cells, causing SOD, GSH-Px levels increased and MDA levels decreased.

CONCLUSION: Salidroside inhibited H₂O₂ induced HLEC injury by decreasing the intracellular MDA content levels and increasing SOD, GSH-Px content levels, which conclude that salidroside may have a certain role in the treatment of HLEC damage.