氢对氧化应激诱导的视网膜衰老的保护机制
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国家自然科学基金资助项目(No.31571184); 辽宁省科技厅-锦州医科大学联合基金项目(No.20170540382)


Protective mechanism of hydrogen on oxidative stress-induced retinal aging
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National Natural Science Foundation of China(No.31571184); Joint Fund Project of Jinzhou Medical University, Liaoning Science and Technology Department(No.20170540382)

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    摘要:

    目的:探讨氢对氧化应激诱导的视网膜衰老的保护机制。

    方法:将小鼠随机分为三组:对照组、模型组(NaIO3处理组)和治疗组(H2水灌胃组)。模型组通过鼠尾静脉注射NaIO3溶液建立视网膜氧化应激损伤模型; 对照组小鼠注射生理盐水; 治疗组予富含H2的饮用水灌胃后造模。利用SA-β-gal染色检测视网膜衰老情况。收集小鼠视网膜,western-blot检测DNA损伤反应相关蛋白ATM、NF-κB蛋白、细胞周期蛋白D1(Cyclin D1)和DNA修复相关蛋白HMGB1的表达。

    结果:SA-β-gal染色显示,治疗组蓝绿色沉淀较模型组相比减少。western-blot结果显示治疗组中DNA损伤反应相关蛋白ATM、NF-κB、Cyclin D1相对表达量(0.10±0.009、0.32±0.01、0.19±0.002)较模型组(0.77±0.08、0.70±0.02、0.36±0.01)均显著降低,差异均具有统计学意义(均P<0.01); 而治疗组中DNA修复相关蛋白HMGB1相对表达量(0.927±0.06)较模型组(0.383±0.07)显著升高,差异有统计学意义(P<0.01)。

    结论:氢可以通过抑制氧化应激诱导的DNA损伤减弱视网膜衰老。

    Abstract:

    AIM: To investigate the protective mechanism of hydrogen on retinal senescence induced by oxidative stress.

    METHODS: Mice were randomly divided into three groups: control group, model group and treatment group. Animal models of retinal oxidative stress injury were established by injecting sodium iodate solution into mice caudal vein. Harvesting the mice retina, Western-blot was used to detect the level of proteins related to DNA damage response such as ATM, NF-κB, cyclin D1 and HMGB1 that associated with DNA repair.

    RESULTS: SA-β-gal staining showed that the blue-green deposits in treatment group were reduced than that in model group. The expression of DNA damage reactive protein in treatment group ATM, cyclin D1, NF-κB(0.10±0.009, 0.32±0.01, 0.19±0.002)were significantly lower than those in the model groups(0.77±0.08, 0.70±0.02, 0.36±0.01), and the differences were statistically significant(all P<0.01). At the same time, the expression of DNA repair protein HMGB1 in treatment group(0.927±0.06)were notably higher than that in model group(0.383±0.07)and the difference was statistically significant(P<0.01).

    CONCLUSION: H2 can attenuate senescence by inhibiting oxidative-stress induced DNA damage.

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李睿婵,刘华,李丽华.氢对氧化应激诱导的视网膜衰老的保护机制.国际眼科杂志, 2019,19(2):200-203.

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  • 收稿日期:2018-07-27
  • 最后修改日期:2019-01-03
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  • 在线发布日期: 2019-01-17
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