目的：探究桑色素滴眼液在围绝经期雌性兔干眼症中的治疗作用。

方法：选取雌性新西兰大白兔36只，采用双侧卵巢切除术，术后饲养60d，制作围绝经期雌兔干眼症模型(36眼，均为右眼)，其中24只随机分成对照组、实验组两组(各12只)：对照组应用磷酸盐缓冲液(PBS)，实验组应用桑色素滴眼液，另12只不使用任何滴眼液。治疗前，治疗后2、4、6wk对实验兔采取角膜荧光素染色(FL)、Schirmer I试验(SⅠt)检查，同时测验溶菌酶含量、乳铁蛋白、淀粉酶活性和泪液总蛋白含量，并使用角膜共聚焦显微镜观察。

结果：治疗前两组的SⅠt、FL、溶菌酶含量、乳铁蛋白、淀粉酶活性和泪液总蛋白量均无差异(P>0.05)； 治疗2、4、6wk后，对照组和实验组SⅠt和FL评分发生了不同的变化(P<0.05)。实验组溶菌酶含量、乳铁蛋白、淀粉酶活性和泪液总蛋白量较治疗前没有显著变化(均P>0.05)，对照组溶菌酶含量、乳铁蛋白、淀粉酶活性和泪液总蛋白量都发生了程度不一的变化(均P<0.05)。治疗后2、4、6wk时两组SⅠt、FL评分、溶菌酶含量、乳铁蛋白、淀粉酶活性和泪液总蛋白量均有差异(P<0.05)。治疗6wk，对照组炎症细胞密度为321±91个/mm²，上皮基底细胞密度为4436±289个/mm²，实验组炎症细胞密度为36±11个/mm²，上皮基底细胞密度为3219±223个/mm²，两组均有差异(P<0.05)。治疗6wk后，对照组角膜上皮下多支神经纤维发生弯曲，密度显著减少(P<0.05)，实验组神经弯曲度明显减小，密度较治疗前增加。

结论：桑色素滴眼液对围绝经期雌性兔干眼症具有明确的治疗作用。

METHODS: Totally 36 female New Zealand white rabbits were selected and operated with bilateral ovariectomy and fed for 60d postoperatively to make perimenopausal female rabbit dry eye model(36 eyes, all right eyes). And 24 of them were randomly divided into control group and experimental group(12 for each): the control group used PBS, the experimental group used Mulberry eye drops, the other 12 did not use any eye drops. The Schirmer I tests(SⅠt)and corneal fluorescein(FL)were made, and the tear total protein content, amylase activity, lactoferrin and lysozyme contents and confocal scanning microscopy were performed in two groups before treatment and 2, 4, 6wk after treatment.

RESULTS: There was no significant difference in SⅠt,FL scores,total proteins,lysozyme,lactoferrin contents and amylase activity between two groups at pre-therapy(all P>0.05).After 2, 4 and 6wk of treatment, there were significant changes in SⅠt and FL scores in the two groups(P<0.05). There were no significant changes in lysozyme content, lactoferrin, amylase activity and total tear protein in the experimental group compared with those before treatment(all P>0.05), while there were significant changes in lysozyme content, lactoferrin, amylase activity and total tear protein in the control group(all P<0.05). At 2, 4 and 6wk after treatment, there were differences in the scores of SⅠt and FL, lysozyme content, lactoferrin, amylase activity and total protein content in tear between the two groups(P<0.05). At 6 wk after treatment, the mean number of corneal basal cells and inflammatory cells in control group were 4436±289mm² and 321±91mm²,respectively,which in experimental group were 3219±223mm² and 36±11mm², respectively,there were statistical differences between two groups( all P<0.05). After 6wk treatment, there were no change of corneal nerve bending and less density in control group while the nerve fiber bending and density decreased significantly in experimental group,there were statistical differences between two groups(all P<0.05).

CONCLUSION: Mulberry eye drops has significantly therapy effects on the dry eye induced by decreased androgen and it has a definite clinical application value.