双光子钙成像技术在模型动物视皮层Ⅱ/Ⅲ层神经元功能研究中的应用

doi:10.3980/j.issn.1672-5123.2020.9.12

首页 > 过刊浏览>2020年第20卷第9期 >2020,20(9):1529-1532. DOI:10.3980/j.issn.1672-5123.2020.9.12

双光子钙成像技术在模型动物视皮层Ⅱ/Ⅲ层神经元功能研究中的应用

李谦
李谦
中国山东省济南市,山东中医药大学
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毕爱玲
毕爱玲
中国山东省济南市,山东中医药大学; 中国山东省济南市,山东省中西医结合眼病防治重点实验室山东省高校中西医结合眼病防治技术(强化)重点实验室山东中医药大学眼科研究所山东中医药大学附属眼科医院
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刘德政
刘德政
中国山东省济南市,山东中医药大学
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毕宏生
毕宏生
中国山东省济南市,山东中医药大学; 中国山东省济南市,山东省中西医结合眼病防治重点实验室山东省高校中西医结合眼病防治技术(强化)重点实验室山东中医药大学眼科研究所山东中医药大学附属眼科医院
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王兴荣
王兴荣
中国山东省济南市,山东中医药大学; 中国山东省济南市,山东省中西医结合眼病防治重点实验室山东省高校中西医结合眼病防治技术(强化)重点实验室山东中医药大学眼科研究所山东中医药大学附属眼科医院
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Roles of two-photon calcium imaging technology for visual cortex Ⅱ/Ⅲ layer neurons in model animals

Qian Li
Qian Li
Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China
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Ai-Ling Bi
Ai-Ling Bi
Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China; Shandong Provincial Key Laboratory of Integrated Traditional Chinese, Western Medicine for Prevention and Therapy of Ocular Diseases Key Laboratory of Integrated Traditional Chinese
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De-Zheng Liu
De-Zheng Liu
Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China
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Hong-Sheng Bi
Hong-Sheng Bi
Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China; Shandong Provincial Key Laboratory of Integrated Traditional Chinese, Western Medicine for Prevention and Therapy of Ocular Diseases Key Laboratory of Integrated Traditional Chinese
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Xing-Rong Wang
Xing-Rong Wang
Shandong University of Traditional Chinese Medicine, Jinan 250014, Shandong Province, China; Shandong Provincial Key Laboratory of Integrated Traditional Chinese, Western Medicine for Prevention and Therapy of Ocular Diseases Key Laboratory of Integrated Traditional Chinese
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发布日期：2020-08-19

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[关键词]

视皮层 ; Ⅱ/Ⅲ层 ; 钙信号 ; 双光子钙成像技术

[摘要]

钙离子是哺乳动物神经细胞内的重要信使，可以介导多种细胞内信号转导通路，在调控神经元的功能方面发挥关键作用。钙信号在明确的细胞亚区发挥其高度特异性的功能，特别是在大脑视皮层中更能反映神经元的活性，因此对神经元进行钙信号的检测对研究视皮层功能尤其重要。双光子显微镜在皮层浅层区域钙信号检测方面具有独特优势。本文综述了双光子在体检测技术在模型动物视皮层Ⅱ/Ⅲ层神经元功能研究中的应用。

[Key word]

visual cortex ; Ⅱ/Ⅲ layer ; calcium signal ; two-photon calcium imaging

[Abstract]

Calcium is an important messenger in the mammalian nerve cells which mediates a variety of intracellular signal transduction pathways and plays critical roles in regulating the neuronal functions. Calcium signaling exerts its highly specific function in a defined sub-region of the cell, especially in the visual cortex of the brain. Detection of calcium signals in neurons is particularly important for the studying of neuronal function. The two-photon microscope has a unique advantage in the detection of calcium signal in the superficial cortex. In this paper, the application of two-photon in the in vivo detection of the visual cortical Ⅱ/Ⅲ layer of model animals are reviewed.

[中图分类号]

[基金项目]

国家重点研发计划(No.2019YFC1710200)

引用本文 / Get Citation

李谦,毕爱玲,刘德政,等.双光子钙成像技术在模型动物视皮层Ⅱ/Ⅲ层神经元功能研究中的应用.国际眼科杂志, 2020,20(9):1529-1532.

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历史 / History

收稿日期: 2019-10-23

最后修改日期: 2020-08-03

在线发布日期: 2020-08-19

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