Hippo signaling pathway plays an important role in cell growth, proliferation, apoptosis, and stem cell regeneration. Dysregulation of this pathway has been linked to various diseases, such as cancer, heart disease, lung disease, kidney disease, liver disease, and immune dysfunction, as well as ocular diseases. Consequently, regulating the balance of Hippo signaling pathway may be an effective treatment for ocular diseases. This article reviews the mechanism of action and therapeutic potential of Hippo signaling pathway in ocular diseases, and discusses the potential therapeutic targets of the Hippo signaling pathway, to identify new targets and provide new research ideas for treating ocular diseases.