目的：探讨p53凋亡刺激蛋白2(ASPP2)对兔外伤性增生性玻璃体视网膜病变(PVR)模型的作用。

方法：选取新西兰白兔30只，右眼作约6 mm的巩膜穿通伤后随机分为实验组与对照组，每组15只，实验组联合玻璃体腔内注入ASPP2慢病毒转染的ARPE-19细胞悬液0.1 mL，对照组联合玻璃体腔内注入阴性对照慢病毒转染的ARPE-19细胞悬液0.1 mL。在造模后1、2、3、4 wk，使用手持眼压计测量眼压； 眼底照相以及眼部B型超声检查检测建模情况。造模后4 wk通过视网膜组织切片及HE染色检测兔眼视网膜的形态变化； Western blot法检测兔视网膜中ASPP2以及上皮-间质转化(EMT)标志物Vimentin的蛋白表达。

结果：在造模后的第1、2、3、4 wk，PVR造模前后实验组及对照组兔眼的组内眼压变化不显著，实验组的PVR成模率低于对照组(P<0.05)，实验组视网膜增殖及结构紊乱较对照组减轻。在造模后4 wk，实验组兔眼视网膜中ASPP2的蛋白表达量较对照组显著升高(t=3.193，P=0.033)，Vimentin的蛋白表达量显著降低(t=-3.599，P=0.023)。

结论：ASPP2参与调节RPE细胞的EMT，延缓兔眼外伤性PVR的发生发展。

METHODS:A total of 30 New Zealand white rabbits were selected, and the right eyes of all rabbits were inflicted with a scleral penetrating wound of approximately 6 mm. Then rabbits were randomly and evenly divided into experimental and control group. The experimental group received an intravitreal injection of 0.1 mL of ARPE-19 cell suspension transfected with lentivirus-ASPP2, while the control group received an intravitreal injection of 0.1 mL of ARPE-19 cell suspension transfected with negative control lentivirus. At 1, 2, 3, and 4 wk after PVR modeling, a handheld tonometer was used to measure the intraocular pressure. Moreover, fundus photography and ocular ultrasound examination were performed to detect the retinal proliferation. At 4 wk after modeling, hematoxylin-eosin staining was used to observe the morphological retinal changes, and Western blot was used to determine the protein expressions of ASPP2 and the epithelial-mesenchymal transition(EMT)marker Vimentin in the rabbit retinas.

RESULTS:At 1, 2, 3, and 4 wk after modeling, there were no significant changes in intraocular pressure within the experimental and control group of rabbit eyes, either before or after PVR modeling, the success rate of PVR modeling in the experimental group was lower than that in the control group(P<0.05), and the retinal proliferation and structural disorder was less severe in the experimental group. At 4 wk after modeling, the retinal protein expression level of ASPP2 in the experimental group was significantly higher than that in the control group(t=3.193, P=0.033), while the Vimentin protein expression level was significantly lower in the experimental group(t=-3.599, P=0.023).

CONCLUSION:ASPP2 may be involved in regulating the process of EMT in retinal pigment epithelial cells, thereby delaying the development and progression of traumatic PVR in rabbit eyes.