AIM:To understand effects of octreotide on vascular endothelial growth factor (VEGF) secretion in cultured A375 melanoma cells.·METHODS:A375 cells in vitro amplificated were co-cultured with octreotide of concentration 5,1 and 0.2mg/L for 24,48 and 72 hours.Whether octreotide affected the proliferation of A375 melanoma cells was tested by MTT methods.The octreotide effects on VEGF secretion in A375 cells were tested according to the ELISA method,and octreotide effects on cultured A375 melanoma cell line proliferation and VEGF secretion were statistically analyzed.·RESULTS:Twenty-four hours after coculture,octreo-tide improved the A375 cell proliferation (F=14.180,P=0.00).The concentration and time of octreotide coculture with A375 cells in vitro affected the VEGF secretion significantly (content factor:F=24.441,P=0.00;time factor:F=127.233,P=0.00).Octreotide of the three concentrations improved VEGF secretion in A375 cells in 24 hours.The higher concentration,the less in crease of VEGF level(One-way analysis of variance:F=19.489,P=0.00),and octreotide decreased VEGF secretion in A375 cells in 72-hour,the inhibiting power accorded with the concentration(One-way analysis of variance:F=16.116,P=0.002).·CONCLUSION:Octreotide inhibition on VEGF secretion in A375 melanoma cells is a long-term trend and in a dose-dependent manner.