AIM:To analyze the effects of application of mitomycin C(MMC) and adjustable suture line on the trabeculectomy.·METHODS:Analysis of 65 eyes of 65 glaucoma cases was conducted.MMC and adjustable suture line were used in the trabeculectomy.The patients were visited for 3 months to 1 year and the eye pressure before and after operation,incisal opening anastomosis,anterior chamber formation,filtering bleb and complication after operation were observed.·RESULTS:Anterior chamber formed in 63 eye (96.9%) one day after operation,with grade Ⅰ shallow anterior chamber in 1 eye (1.5%),grade Ⅱshallow anterior chamber in 1 eye (1.5%).During the 7th day to 10th day after operation,the average eye pressure was 13.6mmHg,14.9mmHg after 6 months and 16.7mmHg after one year.All the eyesight of the patients kept unchanged before and after the operation or improved slightly,and the filtering bleb formed.The adjustable suture line could be taken out of the wound according to the eye pressure,anterior chamber and its filtering within one to two weeks after operation.It is necessary to massage the eye balls to help to form the filtering.In this case the eye pressure could be dropped to 2-8mmHg.·CONCLUSION:The adjustable suture line and MMC on trabeculectomy could be safely applied and the complication obviously is decreased.It can be applied in the grass-roots level hospitals because of its remarkable curative effect and simple operation.