To observe clinical effects of the amniotic membrane transplantation combined with autologous serum and Natamycin treatment of fungal corneal ulcer. METHODS: Cropsand plantcaused 80 cases of fungal corneal ulcer (80 eyes) were randomly divided into treatment group and control group with 40 cases in each group, the treatment group was added with autologous serum. Corneal wound healing and corneal scar formation was observed in two groups. All patients underwent surgical debridement of ulcers and necrosis of the corneal tissue lesions under a microscope.Ruiji biological (8mm×10mm amniotic membrane) base membrane surface patching was taken on the ulcer surface, and (10mm×15mm) larger biological amniotic membrane was taken to cover it. They were sutured with 100 Nylon lines. The treatment group was added by subconjunctival autologous serum injection after surgery. RESULTS: All 69 eyes of 69 cases had local wound healed. Treatment group was better than the control group at corneal scar formation and average visual acuity 6 months after surgery The difference was statistically significant (P<0.01). CONCLUSION: Multilayer amniotic membrane transplantation plus subconjunctival injection of autologous serum treatment of fungal corneal ulcer is good