AIM:To analyze the predominant bacteria changes in patients with chronic dacryocystitis and antibiotic sensitivity.·METHODS:Material of lacrimal secretion in 40 cases of patients with chronic dacryocystitis was sampled for bacterial culture,isolation,identification and drug sensitivity test.·RESULTS:Of the 40 cases of chronic dacryocystitis patients with lacrimal secretion culture,39 cases were positive,a total of 50 strains positive bacteria were isolated.There were 27 strains of G+ cocci (54%) with 22 strains of Staphylococcus epidermidis (44%) and G-bacilli 14 strains(28%).Staphylococcus epidermidis sensitive to rifampin was 91%,levofloxacin 66% and ofloxacin 60%.·CONCLUSION:G+ cocci is the main bacteria of chronic dacryocystitis including Staphylococcus epidermidis as the predominant bacteria.We should pay attention to the new uses of old drugs,and changes in first-line drug resistance,combination with drug sensitivity results for reasonable selection of antibiotics.