·AIM:To investigate the change of p21,CDK2 and Smad4 on the HLEC transfected with pEGFP-TGF-β1 plasmid and the effect of TGF-β1gene transfection on the cell cycle control of human lens epithelial cell.·METHODS:Immortalized human lens epithelial cellline(HLEC-B3)was transfered by pEGFP-TGF-β1 gene.The expression of TGF-β1mRNA,p21mRNA,CDK2mRNA and Smad4 mRNA and protein induced by transfered by TGF-β1 gene were measured by RT-PCR and Western-blot at different time periods.·RESULTS:RT-PCR detected that TGF-β1mRNA began to raise after 24h,culminated after 48h,decreased after 72h,decreased much after 96h,but still more than the normal.p21mRNA companied with it.but CDK2 decreased with it.Western-blot detected that the consequence consisted with RT-PCR.The effect of TGF-β1 gene was demonstrated from the lever of transcription and translation.Smad4 protein raised with the lever of TGF-β1 gene at various time point,but disappeared very quickly.·CONCLUSION:TGF-β1 can increase p21,but decrease CDK2,to impact the cell cycle of HLEC.TGF-β/Smad4 pathway take part in the course.·