To explore the changes of corneal endothelium after the Nd∶YAG and 532nm laser therapy. METHODS: The corneal endothelia of the eyes which had been subjected to the treatment of peripheral iridectomy by using the Nd∶YAG laser and those subjected to the treatment by using the 532nm laser were observed with corneal endothelium microscope. RESULTS: Among the entire Nd∶YAG laser sufferers 14 days later, in the center of the corneal endothelium, the decrease of the cell density(CD) revealed statistically significant difference (P<0.05). After the treatment of peripheral iridectomy 14 days later, in the center of the corneal endothelium, the decrease of the CD revealed statistically significant difference (P<0.05). 7 days later, in the periphery of the corneal endothelium, average cell area,minimum cell area, CD and percentage of hexagonal endothelium(6A) were analyzed with statistically significant difference (P<0.05). There was no significant correlation between pre- and postoperative central and 6 oclock periphery of corneal endothelium in central serous chorioretinopathy(CSC). CONCLUSION:The Nd∶YAG laser peripheral iridectomy has damage to corneal endothelium. The treatment of CSC by using the 532nm laser has no statistically significant damage to the corneal endothelium