AIM: To observe the long-term effect and complication of biological amnion combined with Ahmed glaucoma valve implanta-tion on neovascular glaucoma. METHODS: This was a retrospective observational case series. Patients with neovascular glaucoma underwent either of the two procedures from Jan. 2008 Sept. 2009. Twenty-five cases 25 eyes underwent biological amnion combined with Ahmed glaucoma valve implan-tation,while twenty-three cases 23 eyes underwent Ahmed glaucoma valve implantation. RESULTS: The success rate of biological amnion combined with Ahmed glaucoma valve implantation was 96% at the time of discharge from hospital and 88% 3 months later, 83% 12 months later; while the rate of Ahmed glaucoma valve implantation were 91% at the time of discharge from hospital and 59% 3 months later, 55% 12 months later. The long-term effects of the two groups had statistical difference (P<0.05). CONCLUSION: It is considered the biological amnion combined with Ahmed glaucoma valve implantation surgery may be effective and safe for treating neovascular glaucoma .