·AIM:To study the expression and distribution of MUC5AC,keratin 7,p63 and proliferating cell nuclear antigen(PCNA)of adult conjunctival epithelial cells to investigate the localization characteristics of adult conjunctival epithelial stem cells.·METHODS:Conjunctiva was collected from donor of corneal transplantation.HE immunohistochemical staining were performed to observe the expression and distribution of MUC5AC,keratin 7,p63 and PCNA.·RESULTS:PCNA and p63 staining showed similar expression pattern,mainly in the deep layer nucleus of palpebral mucocutaneous junction and limbal region.Keratin 7 was positive expressed in the gland cells of lamina propria in the fornix region.MUC5AC was positive expressed in the cytoplasm of goblet cells in various parts of conjunctival epithelium.·CONCLUSION:Conjunctival stem cells with high capability of proliferation are mainly located in mucocutaneous junction area and limbus in adult.·