·AIM:To determine the protection of procyanidins(PC)against UVB-induced oxidative damage of lens epithelial cells(LECs)with alkaline single cell gel electrophoresis,in order to provide an experimental foundation for cataract clinical treatment.·METHODS:LECs lines of generation 4 were divided randomly into 5 groups.As untreated group:normal cultur-ed LECs;control group:LECs + UVB;three treated groups:PC treated groups:LECs + PC + UVB;taurine(TAU)treat-ed groups:LECs + TAU + UVB;VC treated groups:LECs+VC+UVB.All groups of antioxidants were divided into 3 groups according to the final concentration from low to high(0.05,0.1,0.2mg/mL).Each treated group was treated by antioxidants 2 hours before irradiated under UVB,respectively,then conventional cultivation.The LECs of treated groups and control group were all irradiated at UVB-dose 15mJ/cm2.All of LECs in every group were collected after treatment an hour respectively and analyzed with alkaline single cell gel electrophoresis.·RESULTS:The differences of tail length,percentage of tail DNA and tail moment between PC treated groups and control group were statistically significant(P<0.01).The differences of percentage of tail DNA,tail moment in each PC treated group and untreated group were not significant(P>0.05).The results of PC treated groups were signi-ficantly smaller than those of TAU treated groups and VC treated groups,The differences of tail length,percent-age of tail DNA and tail moment in various antioxidant treated groups were statistically significant(P<0.01).·CONCULSION:Exogenetic PC has protective effect to UVB-induced LECs DNA damage.The protective effect of PC is obviously stronger than those of TAU and VC,and in a certain range of concentration,the protective effect of PC has the positive relationship of dose-effect.·