AIM:To analyze the expression changes of tumor necrosis factor α (TNF-α),interleukin-6 (IL-6) and interleukin-8 (IL-8) in conjunctivochalasis tears. ·METHODS:Tears samples were obtained from 25 conjunctivochalasis cases and 13 normal controls. Fifteen microliters of tears were collected by microcapillary tubes from each eye. The tear concentrations of TNF-α,IL-6 and IL-8 were analyzed by radioimmunoassay method and compared the statistical difference between conjunctivochalasis and normal controls. ·RESULTS:The concentration of TNF-α in conjunctiv-ochalasispatients was 19.05±6.35ng/mL,in normal control group was 14.13±7.76ng/mL; conjunctivochalasis concentration of IL-6 was 13.16±8.97pg/mL,normal control group concentration of IL-6 was 4.24±3.97pg/mL; conjunctivochalasis concentration of IL-8 was 34.40±20.73pg/mL,normal control group concentration of IL-8 was 18.31±11.62pg/mL. The difference between the two groups was significant (P<0.05). ·CONCLUSION:Radioimmunoassay method could detect levels of cytokines in tears. The tear concentrations of the three cytokines from the conjunctivochalasis group were significantly higher,which may indicate conjunctivochalasis is related to the increase of cytokines and immune response.