AIM:To evaluate the clinical effect of trabeculectomy combined with bio-amnion implantation for patients with refractory glaucoma. METHODS:Totally 62 eyes of 46 glaucoma patients were randomly divided into 2 groups.In group A,32 eyes underwent trabeculectomy combined with bio-amnion implantation.In group B,30 eyes were as control group. The intraocular pressure(IOP),filtrative bleb,visual acuity and complications were observed. RESULTS:Following-up 12 months,the postoperative conditions were compared with those before the surgery. The IOP of both groups was significantly declined(P<0.01).The average IOP in group A was about 12.3±2.3mmHg,while in group B was about 15.7±2.7mmHg. 88%wasⅠandⅡbleb in group A,67%wasⅠandⅡbleb in group B.There was significant difference between two groups(P<0.05).Postoperative complications:shallow anterior chamber;5 eyes in group A,3 eyes in group B; choroidal detachment:2 eyes in group A,2 eyes in group B;bleb leaking:1 eye in group B. CONCLUSION:Combined trabeculectomy with bioamnion implantation can increase the success ratio in refractory glaucoma surgery.